Mutational analysis of Burkholderia thailandensis quorum sensing and self-aggregation.

ABSTRACT: Acyl-homoserine lactone (acyl-HSL) quorum-sensing signaling is common to many Proteobacteria. Acyl-HSLs are synthesized by the LuxI family of synthases, and the signal response is mediated by members of the LuxR family of transcriptional regulators. Burkholderia thailandensis is a member of a closely related cluster of three species, including the animal pathogens Burkholderia mallei and Burkholderia pseudomallei. Members of this group have similar luxI and luxR homologs, and these genes contribute to B. pseudomallei and B. mallei virulence. B. thailandensis possesses three pairs of luxI-luxR homologs. One of these pairs, BtaI2-BtaR2, has been shown to produce and respond to 3OHC(10)-HSL and to control the synthesis of an antibiotic. By using a markerless-exhange method, we constructed an

SUBMITTER: Chandler JR 

PROVIDER: S-EPMC2747893 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature