Microarray and functional analysis of growth phase-dependent gene regulation in Bordetella bronchiseptica.

ABSTRACT: Growth phase-dependent gene regulation has recently been demonstrated to occur in Bordetella pertussis, with many transcripts, including known virulence factors, significantly decreasing during the transition from logarithmic to stationary-phase growth. Given that B. pertussis is thought to have derived from a Bordetella bronchiseptica-like ancestor, we hypothesized that growth phase-dependent gene regulation would also occur in B. bronchiseptica. Microarray analysis revealed and quantitative real-time PCR (qRT-PCR) confirmed that growth phase-dependent gene regulation occurs in B. bronchiseptica, resulting in prominent temporal shifts in global gene expression. Two virulence phenotypes associated with these gene expression changes were tested. We found that growth-dependent increases in e

SUBMITTER: Nicholson TL 

PROVIDER: S-EPMC2747918 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature