Community-wide response of the gut microbiota to enteropathogenic Citrobacter rodentium infection revealed by deep sequencing.

ABSTRACT: We investigated the spatial and temporal response of the murine gut microbiome to infection with Citrobacter rodentium, an attaching-and-effacing bacterium that provokes innate and adaptive immune responses, resulting in transient bacterial colitis. Previous studies have suggested that C. rodentium-induced inflammation is associated with an increased abundance of Enterobacteriaceae. We report here a deeper analysis of this model using DNA bar coding and 454 pyrosequencing to characterize 101,894 partial 16S rRNA gene sequences from 85 microbial samples from tissue-adhered and luminal bacteria of the cecum, proximal colon, and distal colon, which allowed us to identify previously unappreciated spatial and kinetic changes in multiple bacterial lineages. The deep sequencing data revealed that

SUBMITTER: Hoffmann C 

PROVIDER: S-EPMC2747949 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature