A modified TILLING approach to detect induced mutations in tetraploid and hexaploid wheat.

ABSTRACT:

Background

Wheat (Triticum ssp.) is an important food source for humans in many regions around the world. However, the ability to understand and modify gene function for crop improvement is hindered by the lack of available genomic resources. TILLING is a powerful reverse genetics approach that combines chemical mutagenesis with a high-throughput screen for mutations. Wheat is specially well-suited for TILLING due to the high mutation densities tolerated by polyploids, which allow for very efficient screens. Despite this, few TILLING populations are currently available. In addition, current TILLING screening protocols require high-throughput genotyping platforms, limiting their use.

Results

We developed mutant populations of pasta and common wheat and organized them for TILL

SUBMITTER: Uauy C 

PROVIDER: S-EPMC2748083 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature