Genome-wide analysis of signal peptide functionality in Lactobacillus plantarum WCFS1.

ABSTRACT:

Background

Lactobacillus plantarum is a normal, potentially probiotic, inhabitant of the human gastrointestinal (GI) tract. The bacterium has great potential as food-grade cell factory and for in situ delivery of biomolecules. Since protein secretion is important both for probiotic activity and in biotechnological applications, we have carried out a genome-wide experimental study of signal peptide (SP) functionality.

Results

We have constructed a library of 76 Sec-type signal peptides from L. plantarum WCFS1 that were predicted to be cleaved by signal peptidase I. SP functionality was studied using staphylococcal nuclease (NucA) as a reporter protein. 82% of the SPs gave significant extracellular NucA activity. Levels of secreted NucA varied by a dramatic 1800-fold and this

SUBMITTER: Mathiesen G 

PROVIDER: S-EPMC2748100 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature