Virologic characteristics of hepatitis B virus in patients infected via maternal-fetal transmission.

ABSTRACT:

Aim

To determine whether HBV with the same characteristics causes dissimilar mutations in different hosts.

Methods

Full-length HBV genome was amplified and linked with pMD T18 vector. Positive clones were selected by double-restriction endonuclease digestion (EcoRI and HindIII) and PCR. Twenty seven clones were randomly selected from an asymptomatic mother [at two time points: 602 (1 d) and 6022 (6 mo)] and her son [602 (S)], and the phylogenetic and mutational analysis was performed using BioEditor, Clustal X and MEGA software. Potential immune epitopes were determined by the Stabilized Matrix Method (SMM), SMM-Align Method and Emini Surface Accessibility Prediction.

Results

All of the 27 sequences were genotype C, the divergence between the mother and son was 0%-0.8

SUBMITTER: Shen T 

PROVIDER: S-EPMC2748201 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature