Skin electroporation: effects on transgene expression, DNA persistence and local tissue environment.

ABSTRACT:

Background

Electrical pulses have been used to enhance uptake of molecules into living cells for decades. This technique, often referred to as electroporation, has become an increasingly popular method to enhance in vivo DNA delivery for both gene therapy applications as well as for delivery of vaccines against both infectious diseases and cancer. In vivo electrovaccination (gene delivery followed by electroporation) is currently being investigated in several clinical trials, including DNA delivery to healthy volunteers. However, the mode of action at molecular level is not yet fully understood.

Methodology/principal findings

This study investigates intradermal DNA electrovaccination in detail and describes the effects on expression of the vaccine antigen, plasmid persistenc

SUBMITTER: Roos AK 

PROVIDER: S-EPMC2748717 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature