Effects of impurities on membrane-protein crystallization in different systems.

ABSTRACT: When starting a protein-crystallization project, scientists are faced with several unknowns. Amongst them are these questions: (i) is the purity of the starting material sufficient? and (ii) which type of crystallization experiment is the most promising to conduct? The difficulty in purifying active membrane-protein samples for crystallization trials and the high costs associated with producing such samples require an extremely pragmatic approach. Additionally, practical guidelines are needed to increase the efficiency of membrane-protein crystallization. In order to address these conundrums, the effects of commonly encountered impurities on various membrane-protein crystallization regimes have been investigated and it was found that the lipidic cubic phase (LCP) based crystallization meth

SUBMITTER: Kors CA 

PROVIDER: S-EPMC2748966 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature