Identification of Achaete-scute complex-like 1 (ASCL1) target genes and evaluation of DKK1 and TPH1 expression in pancreatic endocrine tumours.

ABSTRACT:

Background

ASCL1 role in pancreatic endocrine tumourigenesis has not been established. Recently it was suggested that ASCL1 negatively controls expression of the Wnt signalling antagonist DKK1. Notch signalling regulates expression of TPH1, the rate limiting enzyme in the biosynthesis of serotonin. Understanding the development and proliferation of pancreatic endocrine tumours (PETs) is essential for the development of new therapies.

Methods

ASCL1 target genes in the pancreatic endocrine tumour cell line BON1 were identified by RNA interference and microarray expression analysis. Protein expressions of selected target genes in PETs were evaluated by immunohistochemistry.

Results

158 annotated ASCL1 target genes were identified in BON1 cells, among them DKK1 and TPH1 t

SUBMITTER: Johansson TA 

PROVIDER: S-EPMC2749870 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature