Regulation and function of FTO mRNA expression in human skeletal muscle and subcutaneous adipose tissue.

ABSTRACT:

Objective

Common variants in FTO (the fat mass- and obesity-associated gene) associate with obesity and type 2 diabetes. The regulation and biological function of FTO mRNA expression in target tissue is unknown. We investigated the genetic and nongenetic regulation of FTO mRNA in skeletal muscle and adipose tissue and their influence on in vivo glucose and fat metabolism.

Research design and methods

The FTO rs9939609 polymorphism was genotyped in two twin cohorts: 1) 298 elderly twins aged 62-83 years with glucose tolerance ranging from normal to type 2 diabetes and 2) 196 young (25-32 years) and elderly (58-66 years) nondiabetic twins examined by a hyperinsulinemic-euglycemic clamp including indirect calorimetry. FTO mRNA expression was determined in subcutaneous adipose ti

SUBMITTER: Grunnet LG 

PROVIDER: S-EPMC2750213 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature