Promoter analysis of the rabbit POU5F1 gene and its expression in preimplantation stage embryos.

ABSTRACT:

Background

The POU5F1 gene encodes the octamer-binding transcription factor-4 (Oct4). It is crucial in the regulation of pluripotency during embryonic development and widely used as molecular marker of embryonic stem cells (ESCs). The objective of this study was to identify and to analyse the promoter region of rabbit POU5F1 gene; furthermore to examine its expression pattern in preimplantation stage rabbit embryos.

Results

The upstream region of rabbit POU5F1 was subcloned sequenced and four highly conserved promoter regions (CR1-4) were identified. The highest degree of similarity on sequence level was found among the conserved domains between rabbit and human. Among the enhancers the proximal enhancer region (PE-1A) exhibited the highest degree of homology (96.4%). Furthe

SUBMITTER: Kobolak J 

PROVIDER: S-EPMC2751759 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature