Mucin-type O-glycans in tears of normal subjects and patients with non-Sjogren's dry eye.

ABSTRACT:

Purpose

O-linked carbohydrates (O-glycans) contribute to the hydrophilic character of mucins in mucosal tissues. This study was conducted to identify the repertoire of O-glycans in the tear film and the glycosyltransferases associated with their biosynthesis, in normal subjects and patients with non-Sjögren's dry eye.

Methods

Human tear fluid was collected from the inferior conjunctival fornix. O-glycans were released by hydrazinolysis, labeled with 2-aminobenzamide, and analyzed by fluorometric, high-performance liquid chromatography (HPLC) coupled with exoglycosidase digestions. O-glycan structures identified in tears were related to potential biosynthetic pathways in human conjunctival epithelium by using a glycogene microarray database. Lectin-binding analyses were perfo

SUBMITTER: Guzman-Aranguez A 

PROVIDER: S-EPMC2751810 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature