Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury.

ABSTRACT:

Aim

To study the role of mitochondrial energy disorder in the pathogenesis of ethanol-induced gastric mucosa injury.

Methods

Wistar rats were used in this study. A gastric mucosal injury model was established by giving the rats alcohol. Gross and microscopic appearance of gastric mucosa and ultrastructure of mitochondria were evaluated. Malondiadehyde (MDA) in gastric mucosa was measured with thiobarbituric acid. Expression of ATP synthase (ATPase) subunits 6 and 8 in mitochondrial DNA (mtDNA) was determined by reverse transcription polymerase chain reaction (RT-PCR).

Results

The gastric mucosal lesion index was correlated with the MDA content in gastric mucosa. As the concentration of ethanol was elevated and the exposure time to ethanol was extended, the content of

SUBMITTER: Pan JS 

PROVIDER: S-EPMC2751896 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature