Microarray-based multicycle-enrichment of genomic subsets for targeted next-generation sequencing.

ABSTRACT: The lack of efficient high-throughput methods for enrichment of specific sequences from genomic DNA represents a key bottleneck in exploiting the enormous potential of next-generation sequencers. Such methods would allow for a systematic and targeted analysis of relevant genomic regions. Recent studies reported sequence enrichment using a hybridization step to specific DNA capture probes as a possible solution to the problem. However, so far no method has provided sufficient depths of coverage for reliable base calling over the entire target regions. We report a strategy to multiply the enrichment performance and consequently improve depth and breadth of coverage for desired target sequences by applying two iterative cycles of hybridization with microfluidic Geniom biochips. Using this str

SUBMITTER: Summerer D 

PROVIDER: S-EPMC2752126 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature