Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection.

ABSTRACT:

Purpose

We have previously shown that a transcriptome is found in saliva and subpanels of these mRNAs can be used as oral cancer biomarkers. In this study, we measured the presence of microRNAs (miRNA) in saliva and determined their potential as an additional set of oral cancer biomarkers.

Experimental design

A total of 314 miRNAs were measured using reverse transcriptase-preamplification-quantitative PCR in 12 healthy controls. Degradation pattern of endogenous and exogenous saliva miRNAs were measured at room temperature over time. Selected miRNAs were validated in saliva of 50 oral squamous cell carcinoma patients and 50 healthy matched control subjects.

Results

We detected approximately 50 miRNAs in both the whole and supernatant saliva. Endogenous saliva miRNA de

SUBMITTER: Park NJ 

PROVIDER: S-EPMC2752355 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature