A novel hybrid yeast-human network analysis reveals an essential role for FNBP1L in antibacterial autophagy.

ABSTRACT: Autophagy is a conserved cellular process required for the removal of defective organelles, protein aggregates, and intracellular pathogens. We used a network analysis strategy to identify novel human autophagy components based upon the yeast interactome centered on the core yeast autophagy proteins. This revealed the potential involvement of 14 novel mammalian genes in autophagy, several of which have known or predicted roles in membrane organization or dynamics. We selected one of these membrane interactors, FNBP1L (formin binding protein 1-like), an F-BAR-containing protein (also termed Toca-1), for further study based upon a predicted interaction with ATG3. We confirmed the FNBP1L/ATG3 interaction biochemically and mapped the FNBP1L domains responsible. Using a functional RNA interfere

SUBMITTER: Huett A 

PROVIDER: S-EPMC2752416 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature