Small molecular weight proteins/peptides present in the in vivo formed human acquired enamel pellicle.

ABSTRACT:

Objective

The aim of this study was to investigate the type and the nature of peptides present in the in vivo formed human acquired enamel pellicle.

Design

Pellicle material was collected from 10 volunteers and subjected to sample preparations consisting of centrifugal filtration using a 10 kDa molecular weight cut-off membrane and high-resolution gel filtration chromatography. The fractions containing peptides <10 kDa obtained by both methods were analyzed by LC-ESI-MS/MS.

Results

78 natural pellicle peptides with molecular weights ranging from 766.9 Da to 3981.4 Da were identified originating from 29 different proteins.

Conclusions

The number of peptides present in acquired enamel pellicle appears to be large and this is likely to enhance the functional spect

SUBMITTER: Siqueira WL 

PROVIDER: S-EPMC2752433 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature