Acetylation by the transcriptional coactivator Gcn5 plays a novel role in co-transcriptional spliceosome assembly.

ABSTRACT: In the last several years, a number of studies have shown that spliceosome assembly and splicing catalysis can occur co-transcriptionally. However, it has been unclear which specific transcription factors play key roles in coupling splicing to transcription and the mechanisms through which they act. Here we report the discovery that Gcn5, which encodes the histone acetyltransferase (HAT) activity of the SAGA complex, has genetic interactions with the genes encoding the heterodimeric U2 snRNP proteins Msl1 and Lea1. These interactions are dependent upon the HAT activity of Gcn5, suggesting a functional relationship between Gcn5 HAT activity and Msl1/Lea1 function. To understand the relationship between Gcn5 and Msl1/Lea1, we carried out an analysis of Gcn5's role in co-transcriptional recru

SUBMITTER: Gunderson FQ 

PROVIDER: S-EPMC2752994 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature