Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis.

ABSTRACT:

Background

MicroRNAs (miRNAs) have critical functions in various biological processes. MiRNA profiling is an important tool for the identification of differentially expressed miRNAs in normal cellular and disease processes. A technical challenge remains for high-throughput miRNA expression analysis as the number of miRNAs continues to increase with in silico prediction and experimental verification. Our study critically evaluated the performance of a novel miRNA expression profiling approach, quantitative RT-PCR array (qPCR-array), compared to miRNA detection with oligonucleotide microchip (microarray).

Results

High reproducibility with qPCR-array was demonstrated by comparing replicate results from the same RNA sample. Pre-amplification of the miRNA cDNA improved sensitivit

SUBMITTER: Chen Y 

PROVIDER: S-EPMC2753550 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature