Genome-wide analysis of Tol2 transposon reintegration in zebrafish.

ABSTRACT:

Background

Tol2, a member of the hAT family of transposons, has become a useful tool for genetic manipulation of model animals, but information about its interactions with vertebrate genomes is still limited. Furthermore, published reports on Tol2 have mainly been based on random integration of the transposon system after co-injection of a plasmid DNA harboring the transposon and a transposase mRNA. It is important to understand how Tol2 would behave upon activation after integration into the genome.

Results

We performed a large-scale enhancer trap (ET) screen and generated 338 insertions of the Tol2 transposon-based ET cassette into the zebrafish genome. These insertions were generated by remobilizing the transposon from two different donor sites in two transgenic lines. We

SUBMITTER: Kondrychyn I 

PROVIDER: S-EPMC2753552 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature