Contribution of epithelial-derived fibroblasts to bleomycin-induced lung fibrosis.

ABSTRACT:

Rationale

Lung fibroblasts are key mediators of fibrosis resulting in accumulation of excessive interstitial collagen and extracellular matrix, but their origins are not well defined.

Objectives

We aimed to elucidate the contribution of lung epithelium-derived fibroblasts via epithelial-mesenchymal transition (EMT) in the intratracheal bleomycin model.

Methods

Primary type II alveolar epithelial cells were cultured from Immortomice and exposed to transforming growth factor-beta(1) and epidermal growth factor. Cell fate reporter mice that permanently mark cells of lung epithelial lineage with beta-galactosidase were developed to study EMT, and bone marrow chimeras expressing green fluorescent protein under the control of the fibroblast-associated S100A4 promoter were g

SUBMITTER: Tanjore H 

PROVIDER: S-EPMC2753790 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature