Mre11S--a yeast mutation that blocks double-strand-break processing and permits nonhomologous synapsis in meiosis.

ABSTRACT: During meiotic prophase the repair of self-inflicted DNA double-strand break (DSB) damage leads to meiotic recombination in yeast. We employed a genetic screen to specifically characterize cellular functions that become essential after this DSB formation. As a result a new allele of MRE11, termed mre11S (for Separation of functions) was isolated that allows initiation but not processing and repair of meiotic DSBs similar to the well-characterized rad50S allele. In contrast, the mre11-1 allele blocks initiation of meiotic DSBs as reported previously by others. The mre11S allele, which is mutated in the 5' part of the gene, can partially complement mre11 alleles disrupted close to the 3' end that cannot initiate DSBs when homozygous. This suggests homodimerization of the Mre11 protein and th

SUBMITTER: Nairz K 

PROVIDER: S-EPMC275393 | biostudies-literature | 1997 Sep

REPOSITORIES: biostudies-literature