Inactivating the spindle checkpoint kinase Bub1 during embryonic development results in a global shutdown of proliferation.

ABSTRACT:

Background

Bub1 is a component of the spindle assembly checkpoint, a surveillance mechanism that maintains chromosome stability during M-phase. Bub1 is essential during the early stages of embryogenesis, with homozygous BUB1-null mice dying shortly after day E3.5. Bub1 is also required later during embryogenesis; inactivation of BUB1 on day E10.5 appears to rapidly block all further development. However, the mechanism(s) responsible for this phenotype remain unclear.

Findings

Here we show that inactivating BUB1 on day E10.5 stalls embryogenesis within 48 hours. This is accompanied by a global shutdown of proliferation, widespread apoptosis and haemorrhaging.

Conclusion

Our results suggest that Bub1 is required throughout the developing embryo for cellular proliferatio

SUBMITTER: Tilston V 

PROVIDER: S-EPMC2754486 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature