Identification of a nuclear localization motif in the serine/arginine protein kinase PSRPK of physarum polycephalum.

ABSTRACT:

Background

Serine/arginine (SR) protein-specific kinases (SRPKs) are conserved in a wide range of organisms, from humans to yeast. Studies showed that SRPKs can regulate the nuclear import of SR proteins in cytoplasm, and regulate the sub-localization of SR proteins in the nucleus. But no nuclear localization signal (NLS) of SRPKs was found. We isolated an SRPK-like protein PSRPK (GenBank accession No. DQ140379) from Physarum polycephalum previously, and identified a NLS of PSRPK in this study.

Results

We carried out a thorough molecular dissection of the different domains of the PSRPK protein involved in its nuclear localization. By truncation of PSRPK protein, deletion of and single amino acid substitution in a putative NLS and transfection of mammalian cells, we observed

SUBMITTER: Liu S 

PROVIDER: S-EPMC2754491 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature