A flexible whole-genome microarray for transcriptomics in three-spine stickleback (Gasterosteus aculeatus).

ABSTRACT:

Background

The use of microarray technology for describing changes in mRNA expression to address ecological and evolutionary questions is becoming increasingly popular. Since three-spine stickleback are an important ecological and evolutionary model-species as well as an emerging model for eco-toxicology, the ability to have a functional and flexible microarray platform for transcriptome studies will greatly enhance the research potential in these areas.

Results

We designed 43,392 unique oligonucleotide probes representing 19,274 genes (93% of the estimated total gene number), and tested the hybridization performance of both DNA and RNA from different populations to determine the efficacy of probe design for transcriptome analysis using the Agilent array platform. The majori

SUBMITTER: Leder EH 

PROVIDER: S-EPMC2754499 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature