A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus.

ABSTRACT:

Background

Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. Indeed, nearly 40% of genes in plants encode proteins of unknown function. Functional characterization of these genes is one of the main challenges in modern biology. In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. Full-length cDNA clones facilitate functional analysis of the corresponding genes enabling manipulation of their expression in heterologous systems and the generation of a variety of tagged versions of the native protein. In addition, the development of full-length cDNA sequences

SUBMITTER: Marques MC 

PROVIDER: S-EPMC2754500 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature