Clonality and allelotype analyses of focal nodular hyperplasia compared with hepatocellular adenoma and carcinoma.

ABSTRACT:

Aim

To identify clonality and genetic alterations in focal nodular hyperplasia (FNH) and the nodules derived from it.

Methods

Twelve FNH lesions were examined. Twelve hepatocellular adenomas (HCAs) and 22 hepatocellular carcinomas (HCCs) were used as references. Nodules of different types were identified and isolated from FNH by microdissection. An X-chromosome inactivation assay was employed to describe their clonality status. Loss of heterozygosity (LOH) was detected, using 57 markers, for genetic alterations.

Results

Nodules of altered hepatocytes (NAH), the putative precursors of HCA and HCC, were found in all the FNH lesions. Polyclonality was revealed in 10 FNH lesions from female patients, and LOH was not detected in any of the six FNH lesions examined, the res

SUBMITTER: Cai YR 

PROVIDER: S-EPMC2754518 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature