Differentiation driven changes in the dynamic organization of Basal transcription initiation.

ABSTRACT: Studies based on cell-free systems and on in vitro-cultured living cells support the concept that many cellular processes, such as transcription initiation, are highly dynamic: individual proteins stochastically bind to their substrates and disassemble after reaction completion. This dynamic nature allows quick adaptation of transcription to changing conditions. However, it is unknown to what extent this dynamic transcription organization holds for postmitotic cells embedded in mammalian tissue. To allow analysis of transcription initiation dynamics directly into living mammalian tissues, we created a knock-in mouse model expressing fluorescently tagged TFIIH. Surprisingly and in contrast to what has been observed in cultured and proliferating cells, postmitotic murine cells embedded in th

SUBMITTER: Giglia-Mari G 

PROVIDER: S-EPMC2754661 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature