Regulation of gene expression by estrogen and testosterone in the proximal mouse reproductive tract.

ABSTRACT: The role of estrogen and testosterone in the regulation of gene expression in the proximal reproductive tract is not completely understood. To address this question, mice were treated with testosterone or estradiol, and RNA from the efferent ducts and caput epididymides was processed and hybridized to Affymetrix M430 2.0 microarrays. Analysis of array output identified probe sets in each tissue with altered levels in hormone-treated versus control animals. Hormone treatment efficacy was confirmed by determination of serum hormone levels before and after treatment and by observed changes in transcript levels of previously reported hormone-responsive genes. Tissue-specific hormone sensitivity was observed with 2867 and 3197 probe sets changing significantly in the efferent ducts after estrog

SUBMITTER: Snyder EM 

PROVIDER: S-EPMC2754886 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature