Unique patterns of molecular profiling between human prostate cancer LNCaP and PC-3 cells.

ABSTRACT:

Background

Human prostate cancer LNCaP and PC-3 cell lines have been extensively used to study prostate cancer progression and to develop therapeutic agents. Although LNCaP and PC-3 cells are generally assumed to represent early and late stages of prostate cancer, respectively, there is limited information regarding gene expression patterns between these two cell lines and its relationship to prostate cancer.

Methods

Comprehensive gene expression analysis was performed. Total RNA was isolated from cultured cells and hybridized to Illumina human BeadChips representing 24,526 transcripts. Bioinformatics analysis was applied to identify cell line specific genes as well as biological mechanisms, pathways, and functions related to the genes.

Results

A total of 2,198 genes

SUBMITTER: Dozmorov MG 

PROVIDER: S-EPMC2755240 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature