Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens.

ABSTRACT:

Background

Coccidiosis caused by protozoans of genus Eimeria is a chicken parasitic disease of great economical importance. Conventional disease control strategies depend on vaccination and prophylactic use of anticoccidial drugs. Alternative solution to prevent and treat coccidiosis could be provided by passive immunization using orally delivered neutralizing antibodies. We investigated the possibility to mitigate the parasitic infection by feeding poultry with antibody expressing transgenic crop seeds.

Results

Using the phage display antibody library, we generated a panel of anti-Eimeria scFv antibody fragments with high sporozoite-neutralizing activity. These antibodies were expressed either transiently in agrobacteria-infiltrated tobacco leaves or stably in seeds of tran

SUBMITTER: Zimmermann J 

PROVIDER: S-EPMC2755478 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature