Roles of the pH signaling transcription factor PacC in Wangiella (Exophiala) dermatitidis.

ABSTRACT: To study the function of the PacC transcription factor in Wangiella dermatitidis, a black, polymorphic fungal pathogen of humans with yeast-phase predominance, the PACC gene was cloned, sequenced, disrupted and expressed. Three zinc finger DNA-binding motifs were found at the N-terminus, and a signaling protease cleavage site at the C-terminus. PACC was more expressed at neutral-alkaline pH than at acidic pH. Truncation at about 40 residues of the coding sequence upstream of the conserved protease processing cleavage site of PacC affected growth on a nutrient-rich medium, increased sensitivity to Na(+) stress, decreased yeast growth at neutral-alkaline pH, and repressed hyphal growth on a nutrient-poor medium at 25 degrees C. Truncation at the coding sequence for the conserved signaling pr

SUBMITTER: Wang Q 

PROVIDER: S-EPMC2755521 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature