Paracrine regulation of angiogenesis and adipocyte differentiation during in vivo adipogenesis.

ABSTRACT: With an increasing incidence of obesity worldwide, rational strategies are needed to control adipogenesis. Growth of any tissue requires the formation of a functional and mature vasculature. To gain mechanistic insight into the link between active adipogenesis and angiogenesis, we developed a model to visualize noninvasively and in real time both angiogenesis and adipogenesis using intravital microscopy. Implanted murine preadipocytes induced vigorous angiogenesis and formed fat pads in a mouse dorsal skin-fold chamber. The newly formed vessels subsequently remodeled into a mature network consisting of arterioles, capillaries, and venules, whereas the preadipocytes differentiated into adipocytes as confirmed by increased aP2 expression. Inhibition of adipocyte differentiation by transfecti

SUBMITTER: Fukumura D 

PROVIDER: S-EPMC2755542 | biostudies-literature | 2003 Oct

REPOSITORIES: biostudies-literature