Calcium is involved in formation of high molecular weight adiponectin.

ABSTRACT:

Background

Adiponectin, an adipocyte-specific secretory protein, is known to circulate as different isoforms in the blood stream.

Methods

Using sucrose gradients and Western blotting on nondenaturing gels, adiponectin isoforms were examined in human serum, plasma, adipose tissue, and cells. The medium from human adipose tissue and human and mouse adipocytes were also examined for changes in isoform formation upon treatment with EGTA.

Results

Comparison of adiponectin complexes revealed distinct differences in distribution of high molecular weight (HMW) forms between human serum and plasma, with an apparent difference in molecular weight. Variation in molecular weight suggested a probable dissociation of the HMW isoforms in the presence of EDTA in the plasma. Examinati

SUBMITTER: Banga A 

PROVIDER: S-EPMC2755550 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature