Measurement of neuropeptides in crustacean hemolymph via MALDI mass spectrometry.

ABSTRACT: Neuropeptides are often released into circulatory fluid (hemolymph) to act as circulating hormones and regulate many physiological processes. However, the detection of these low-level peptide hormones in circulation is often complicated by high salt interference and rapid degradation of proteins and peptides in crude hemolymph extracts. In this study, we systematically evaluated three different neuropeptide extraction protocols and developed a simple and effective hemolymph preparation method suitable for MALDI MS profiling of neuropeptides by combining acid-induced abundant protein precipitation/depletion, ultrafiltration, and C(18) micro-column desalting. In hemolymph samples collected from the crab Cancer borealis, several secreted neuropeptides have been detected, including members fro

SUBMITTER: Chen R 

PROVIDER: S-EPMC2756545 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature