Development of a novel peptide microarray for large-scale epitope mapping of food allergens.

ABSTRACT:

Background

The peptide microarray is a novel assay that facilitates high-throughput screening of peptides with a small quantity of sample.

Objective

We sought to use overlapping peptides of milk allergenic proteins as a model system to establish a reliable and sensitive peptide microarray-based immunoassay for large-scale epitope mapping of food allergens.

Methods

A milk peptide microarray was developed by using commercially synthesized peptides (20-mers, 3 offset) covering the primary sequences of alpha(s1)-casein, alpha(s2)-casein, beta-casein, kappa-casein, and beta-lactoglobulin. Conditions for printing and immunolabeling were optimized using a serum pool of 5 patients with milk allergy. Reproducibility of the milk peptide microarray was evaluated using replicate

SUBMITTER: Lin J 

PROVIDER: S-EPMC2757036 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature