Arp2 depletion inhibits sheet-like protrusions but not linear protrusions of fibroblasts and lymphocytes.

ABSTRACT: The Arp2/3 complex-mediated assembly and protrusion of a branched actin network at the leading edge occurs during cell migration, although some studies suggest it is not essential. In order to test the role of Arp2/3 complex in leading edge protrusion, Swiss 3T3 fibroblasts and Jurkat T cells were depleted of Arp2 and evaluated for defects in cell morphology and spreading efficiency. Arp2-depleted fibroblasts exhibit severe defects in formation of sheet-like protrusions at early time points of cell spreading, with sheet-like protrusions limited to regions along the length of linear protrusions. However, Arp2-depleted cells are able to spread fully after extended times. Similarly, Arp2-depleted Jurkat T lymphocytes exhibit defects in spreading on anti-CD3. Interphase Jurkats in suspension a

SUBMITTER: Nicholson-Dykstra SM 

PROVIDER: S-EPMC2757053 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature