Pathogenic bacteria induce colonic PepT1 expression: an implication in host defense response.

ABSTRACT:

Background & aims

Expression of the di/tripeptide transporter PepT1 has been observed in the colon under inflammatory conditions; however, the inducing factors and underlying mechanisms remain unknown. Here, we address the effects of pathogenic bacteria on colonic PepT1 expression together with its functional consequences.

Methods

Human colonic HT29-Cl.19A cells were infected with the attaching and effacing enteropathogenic Escherichia coli (EPEC). Wild-type and PepT1 transgenic mice or cultured colonic tissues derived from these mice were infected with Citrobacter rodentium, a murine attaching and effacing pathogen related to EPEC.

Results

EPEC induced PepT1 expression and activity in HT29-Cl.19A cells by intimately attaching to host cells through lipid rafts. Induct

SUBMITTER: Nguyen HT 

PROVIDER: S-EPMC2757477 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature