APC promoter methylation and protein expression in hepatocellular carcinoma.

ABSTRACT:

Purpose

We investigated the impact of promoter methylation on APC protein expression in patients with hepatocellular carcinoma (HCC).

Materials and methods

50 patients [HCC (n=19), liver metastasis (n=19), cholangiocellular cancer (n=7), and benign liver tumors (n=5)] were studied for methylation using Methylight analysis. APC mutation was investigated by protein truncation test and direct sequencing of genomic DNA. The protein expression was evaluated by immunohistochemistry and Western blot analysis.

Results

The APC promoter was hypermethylated in 81.8% of non-cancerous liver tissue samples. All HCC samples and ten patients with liver metastasis (52.6%) exhibited APC promoter methylation. The degree of methylation was significantly higher in samples from HCC compare

SUBMITTER: Csepregi A 

PROVIDER: S-EPMC2757596 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature