Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases.

ABSTRACT: Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of monosaccharide substrates using engineered Bacillus megaterium cytochrome P450 (P450(BM3)) demethylases that provides a highly efficient means to access valuable intermediates, which can be converted to a wide range of substituted monosaccharides and polysaccharides. Demethylases displaying high levels of regioselectivity toward a number of protected monosaccharides were identified using a combination of protein and substrate engineering, suggesting that this approach ultimately could be used i

SUBMITTER: Lewis JC 

PROVIDER: S-EPMC2757845 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature