Mapping of the functional boundaries and secondary structure of the mouse mammary tumor virus Rem-responsive element.

ABSTRACT: Mouse mammary tumor virus (MMTV) is a complex retrovirus that encodes at least three regulatory and accessory proteins, including Rem. Rem is required for nuclear export of unspliced viral RNA and efficient expression of viral proteins. Our previous data indicated that sequences at the envelope-3' long terminal repeat junction are required for proper export of viral RNA. To further map the Rem-responsive element (RmRE), reporter vectors containing various portions of the viral envelope gene and the 3' long terminal repeat were tested in the presence and absence of Rem in transient transfection assays. A 476-bp fragment that spans the envelope-long terminal repeat junction had activity equivalent to the entire 3'-end of the mouse mammary tumor virus genome, but further deletions at the 5'-

SUBMITTER: Mertz JA 

PROVIDER: S-EPMC2757966 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature