Protein dynamics from NMR: the slowly relaxing local structure analysis compared with model-free analysis.

ABSTRACT: (15)N-(1)H spin relaxation is a powerful method for deriving information on protein dynamics. The traditional method of data analysis is model-free (MF), where the global and local N-H motions are independent and the local geometry is simplified. The common MF analysis consists of fitting single-field data. The results are typically field-dependent, and multifield data cannot be fit with standard fitting schemes. Cases where known functional dynamics has not been detected by MF were identified by us and others. Recently we applied to spin relaxation in proteins the slowly relaxing local structure (SRLS) approach, which accounts rigorously for mode mixing and general features of local geometry. SRLS was shown to yield MF in appropriate asymptotic limits. We found that the experimental spect

SUBMITTER: Meirovitch E 

PROVIDER: S-EPMC2758167 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature