Quantification of cardiovascular biomarkers in patient plasma by targeted mass spectrometry and stable isotope dilution.

ABSTRACT: Verification of candidate biomarkers requires specific assays to selectively detect and quantify target proteins in accessible biofluids. The primary objective of verification is to screen potential biomarkers to ensure that only the highest quality candidates from the discovery phase are taken forward into preclinical validation. Because antibody reagents for a clinical grade immunoassay often exist for a small number of candidates, alternative methodologies are required to credential new and unproven candidates in a statistically viable number of serum or plasma samples. Using multiple reaction monitoring coupled with stable isotope dilution MS, we developed quantitative, multiplexed assays in plasma for six proteins of clinical relevance to cardiac injury. The process described does not

SUBMITTER: Keshishian H 

PROVIDER: S-EPMC2758760 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature