Saffron is a monomorphic species as revealed by RAPD, ISSR and microsatellite analyses.

ABSTRACT:

Background

Saffron (Crocus sativus) is considered the world's most expensive spice. Used mainly as a colorant for foodstuffs, it is highly appreciated for its aromatic and flavouring properties. Since no molecular markers for this species have been found in the literature, the objective of this study was to determine whether phenotypical differences found in C. sativus were supported by molecular analyses.

Findings

Thirty primers from Operon Technologies were used in random amplified polymorphic DNA (RAPD) analysis, forty eight primers were screened using intersimple sequence repeats (ISSR) method and fifteen primers derived from a microsatellites library flanking sequences with repeat motifs were assayed in forty three isolates of C. sativus from eleven different countries

SUBMITTER: Rubio-Moraga A 

PROVIDER: S-EPMC2758891 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature