Ontology and diversity of transcript-associated microsatellites mined from a globe artichoke EST database.

ABSTRACT:

Background

The globe artichoke (Cynara cardunculus var. scolymus L.) is a significant crop in the Mediterranean basin. Despite its commercial importance and its both dietary and pharmaceutical value, knowledge of its genetics and genomics remains scant. Microsatellite markers have become a key tool in genetic and genomic analysis, and we have exploited recently acquired EST (expressed sequence tag) sequence data (Composite Genome Project - CGP) to develop an extensive set of microsatellite markers.

Results

A unigene assembly was created from over 36,000 globe artichoke EST sequences, containing 6,621 contigs and 12,434 singletons. Over 12,000 of these unigenes were functionally assigned on the basis of homology with Arabidopsis thaliana reference proteins. A total of 4,219 p

SUBMITTER: Scaglione D 

PROVIDER: S-EPMC2760586 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature