Mir-214-dependent regulation of the polycomb protein Ezh2 in skeletal muscle and embryonic stem cells.

ABSTRACT: Polycomb group (PcG) proteins exert essential functions in the most disparate biological processes. The contribution of PcG proteins to cell commitment and differentiation relates to their ability to repress transcription of developmental regulators in embryonic stem (ES) cells and in committed cell lineages, including skeletal muscle cells (SMC). PcG proteins are preferentially removed from transcribed regions, but the underlying mechanisms remain unclear. Here, PcG proteins are found to occupy and repress transcription from an intronic region containing the microRNA miR-214 in undifferentiated SMC. Differentiation coincides with PcG disengagement, recruitment of the developmental regulators MyoD and myogenin, and activation of miR-214 transcription. Once transcribed, miR-214 negatively f

SUBMITTER: Juan AH 

PROVIDER: S-EPMC2761245 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature