Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa.

ABSTRACT:

Background

'Systems-wide' approaches such as microarray RNA-profiling are ideally suited to the study of the complex overlapping responses of plants to biotic and abiotic stresses. However, commercial microarrays are only available for a limited number of plant species and development costs are so substantial as to be prohibitive for most research groups. Here we evaluate the use of cross-hybridisation to Affymetrix oligonucleotide GeneChip(R) microarrays to profile the response of the banana (Musa spp.) leaf transcriptome to drought stress using a genomic DNA (gDNA)-based probe-selection strategy to improve the efficiency of detection of differentially expressed Musa transcripts.

Results

Following cross-hybridisation of Musa gDNA to the Rice GeneChip(R) Genome Array, ~33,70

SUBMITTER: Davey MW 

PROVIDER: S-EPMC2761422 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature