2-DE analysis indicates that Acinetobacter baumannii displays a robust and versatile metabolism.

ABSTRACT:

Background

Acinetobacter baumannii is a nosocomial pathogen that has been associated with outbreak infections in hospitals. Despite increasing awareness about this bacterium, its proteome remains poorly characterised, however recently the complete genome of A. baumannii reference strain ATCC 17978 has been sequenced. Here, we have used 2-DE and MALDI-TOF/TOF approach to characterise the proteome of this strain.

Results

The membrane and cytoplasmatic protein extracts were analysed separately, these analyses revealed the reproducible presence of 239 and 511 membrane and cytoplamatic protein spots, respectively. MALDI-TOF/TOF characterisation identified a total of 192 protein spots (37 membrane and 155 cytoplasmatic) and revealed that the identified membrane proteins were mainl

SUBMITTER: Soares NC 

PROVIDER: S-EPMC2761859 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature