Discovery and identification of potential biomarkers of papillary thyroid carcinoma.

ABSTRACT:

Background

Thyroid carcinoma is the most common endocrine malignancy and a common cancer among the malignancies of head and neck. Noninvasive and convenient biomarkers for diagnosis of papillary thyroid carcinoma (PTC) as early as possible remain an urgent need. The aim of this study was to discover and identify potential protein biomarkers for PTC specifically.

Methods

Two hundred and twenty four (224) serum samples with 108 PTC and 116 controls were randomly divided into a training set and a blind testing set. Serum proteomic profiles were analyzed using SELDI-TOF-MS. Candidate biomarkers were purified by HPLC, identified by LC-MS/MS and validated using ProteinChip immunoassays.

Results

A total of 3 peaks (m/z with 9190, 6631 and 8697 Da) were screened out by suppor

SUBMITTER: Fan Y 

PROVIDER: S-EPMC2761863 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature