Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR.

ABSTRACT:

Background

Real-time quantitative reverse transcription PCR (RT-qPCR) data needs to be normalized for its proper interpretation. Housekeeping genes are routinely employed for this purpose, but their expression level cannot be assumed to remain constant under all possible experimental conditions. Thus, a systematic validation of reference genes is required to ensure proper normalization. For soybean, only a small number of validated reference genes are available to date.

Results

A systematic comparison of 14 potential reference genes for soybean is presented. These included seven commonly used (ACT2, ACT11, TUB4, TUA5, CYP, UBQ10, EF1b) and seven new candidates (SKIP16, MTP, PEPKR1, HDC, TIP41, UKN1, UKN2). Expression stability was examined by RT-qPCR across 116 biological sa

SUBMITTER: Hu R 

PROVIDER: S-EPMC2761916 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature